Poly(A) tailing can be used for library preparation of RNAs lacking a natural poly(A) tail, such as miRNA and other small RNAs. This blog discusses the application of poly(A) tailing in RNA-seq.
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Single-Cell RNA Sequencing vs. Single-Nucleus RNA
Single-cell RNA sequencing and single-nucleus RNA sequencing share similar underlying principles, each method offers distinct advantages that suit different research applications
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Introduction to Single-Cell RNA-seq
scRNA-seq measures the distribution of expression levels for each gene at single-cell resolution, enabling researchers to study cellular heterogeneity in unprecedented detail.
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What Is Shotgun Metagenomic Sequencing
Shotgun metagenomic sequencing provides a comprehensive, unbiased approach to microbial community analysis by sequencing all DNA content within a sample.
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Consideration of NGS library prep for FFPE samples
Performing mRNA-seq on FFPE samples is feasible, but presents unique challenges due to degraded and chemically modified RNA
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How to choose the right sequencing technology
Choosing the right sequencing platform depends on factors like project goals, budget, accuracy requirements, and data output.
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Immune repertoire analysis by NGS
Next-generation sequencing (NGS) has revolutionized immune repertoire analysis, enabling researchers to profile millions of TCR/BCR sequences in a single experiment
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Introduction of Next-Generation Sequencing (NGS) Technology
The breakthrough of NGS has transformed fields such as genetic research, precision medicine, cancer genomics, infectious disease surveillance, and agriculture.
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Microbiome Analysis using Next-Generation Sequencing (NGS)
Metagenomics provides researchers with unprecedented insights into microbial diversity, function, and dynamics across various environments - from the human gut to extreme ecosystems
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RNA Sequencing (RNA-Seq) Methods for NGS
RNA sequencing (RNA-Seq) has revolutionized transcriptomics by enabling researchers to analyze gene expression, discover novel transcripts, and detect RNA modifications with high precision.
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The Importance of NGS Sequencing Coverage and Throughput
The quality and reliability of NGS data depend heavily on two critical factors: sequencing coverage and throughput
